Shining Light On Dark Chemical Matter

« Drug Discovery: There’s still hope for compounds that haven’t yet shown biological activity in screening assays, study shows

09342-notw1-HTSrobotcxdA high-throughput screening lab at Novartis.

Some compounds have never shown biological activity, even after being screened repeatedly in many different drug assays. But such “dark chemical matter,” or DCM, might have biological activity that just hasn’t been identified yet. Scientists at pharmaceutical firm Novartis report in a new article that DCM compounds can still be a promising starting point for discovering drug leads (Nat. Chem. Biol. 2015, DOI: 10.1038/nchembio.1936).

“This article asks an important question,” says Paul Clemons of the Broad Institute, who was not involved with this study but has collaborated with some of the researchers. “Just because we haven’t seen any effect, does that mean we will continue failing to see any effect if we keep looking?”

The Novartis team analyzed the results from more than 650 assays, some from the company’s own drug discovery collection and others from a National Institutes of Health program. They found that 14 % of molecules screened in the Novartis assays and 36% of molecules in the NIH assays had never been hits in any test.

The DCM compounds tended to be more soluble and less hydrophobic, with lower molecular weights and with fewer aromatic rings than biologically active compounds. But those physical differences alone don’t explain the lack of activity in DCM molecules, the researchers say.


Exploring chemical space

Substructures highlighted here are common motifs in “dark chemical matter,” a potential source of drug leads.

“Many of the DCM compounds we have identified have no obvious reason that they would, a priori, be inactive in so many of the screens,” says Anne Mai Wassermann, a former postdoc at Novartis who’s now at Pfizer. “These DCM compounds are not dramatically different in structure from compounds that are commonly identified as hits.” Indeed, almost all of the substructural features in “dark” compounds can be found in active compounds, she says.

And just because a compound has not yet been active in a biological assay doesn’t mean that will be the case for all future assays. The Novartis team tested DCM compounds in several of the firm’s assays and identified four DCM compounds with antifungal activity.

“This work has increased the visibility of DCM compounds with respect to how they are viewed as potentially unique or privileged when they hit in an assay,” says John W. Davies, head of in silico lead discovery at Novartis.

“It is our hope that they may be specific to a particular target class or uniquely selective in their mechanism of action,” says Meir Glick, who led the study at Novartis but is now cheminformatics director at Merck & Co.

DCM compounds are on the opposite end of the continuum from a troublesome group of molecules called pan assay interference compounds, or PAINS, which frequently register as hits during assays—and which are often false positives.

“Flagging compounds as DCM may prove useful in high-throughput screening to highlight potential opportunity, just as the noting of compounds as PAINS can be used to highlight potential for severe risk,” says Michael A. Walters, a high-throughput screening expert at the University of Minnesota, Twin Cities.

In screening DCM compounds, “there is a relatively high risk you may not get a hit,” says Jonathan B. Baell, a screening expert at Monash University. “But if you do, the presumed selectivity is then very valuable.”


Dark chemical matter as a promising starting point for drug lead discovery
Anne Mai Wassermann, Eugen Lounkine, Dominic Hoepfner, Gaelle Le Goff, Frederick J King, Christian Studer, John M Peltier, Melissa L Grippo, Vivian Prindle, Jianshi Tao, Ansgar Schuffenhauer, Iain M Wallace, Shanni Chen,Philipp Krastel, Amanda Cobos-Correa, Christian N Parker, John W Davies& Meir Glick
Nature Chemical Biology (2015)
Received 18 February 2015; Accepted 10 September 2015 ; Published online 19 October 2015


« High-throughput screening (HTS) is an integral part of early drug discovery. Herein, we focused on those small molecules in a screening collection that have never shown biological activity despite having been exhaustively tested in HTS assays.

These compounds are referred to as ‘dark chemical matter’ (DCM). We quantified DCM, validated it in quality control experiments, described its physicochemical properties and mapped it into chemical space. Through analysis of prospective reporter-gene assay, gene expression and yeast chemogenomics experiments, we evaluated the potential of DCM to show biological activity in future screens.

We demonstrated that, despite the apparent lack of activity, occasionally these compounds can result in potent hits with unique activity and clean safety profiles, which makes them valuable starting points for lead optimization efforts. Among the identified DCM hits was a new antifungal chemotype with strong activity against the pathogen Cryptococcus neoformans but little activity at targets relevant to human safety. »

Article by Celia Henry Arnaud
Chemical & Engineering News
Volume 93 Issue 42- p. 4 ; Issue Date: October 26, 2015

Cet article n’engage que son auteur/ This article is the sole responsibility of the author