Nasal Cilia: The ‘Long and Short’ of Scent-sation

« Olfactory cilia in the nasal cavity are essential to convert chemical stimuli into electrical responses that, through various steps, transmit odor information to the brain. In relation, researchers at the Perelman School of Medicine at the University of Pennsylvania have identified patterns in cilia length and function within the mouse naval cavity. Their work recently was published in Current Biology and according to the university, may also be implicated in studies related to sight and touch.

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In sensory organs, receptors usually are arranged to enhance detection sensitivity and acuity. Cells along the dorsal recess of the nasal cavity, for example, continuously experience higher air flow rates and odor concentrations. This prompted the team of researchers, led by Minghong Ma, Ph.D., to examine whether cells in different locations evolved unique structural and/or functional features.

Combining fluorescence and electron microscopy, computational fluid dynamics modeling, mouse genetics and electrophysiology, the team found that sensory neurons with longer cilia were positioned for better access to odor molecules and had higher sensitivity.

“We unexpectedly discovered a striking gradient in cilia length, in which cells in the front of the nose have much longer cilia than those in the back, » Ma said, in a press statement. “The discovery that these longer, more sensitive olfactory cilia are located in regions of the nasal cavity where they can detect more molecules may lead to important clues about how such cells operate in other sensory systems.”

As the published paper explains, the higher sensitivity of longer cilia is due to their larger surface area for contacting odors, which gathers larger responses to even low concentrations of odors. Therefore, placing olfactory sensory neurons (OSNs) with longer cilia in highly stimulated regions increases the chances of encountering faint odors and boosts the sensitivity of the system.

In contrast, short-cilia cells have a higher response threshold, making them better suited for coding intensity differences at higher concentrations. In this case, placing OSNs with shorter cilia further back in the nasal cavity, where only stronger odors reach, ensures the broad and dynamic range of the sensory system.

The researchers drew these correlations by simulating eugenol absorption patterns and linking them to cilia length heat maps. Eugenol absorption was found to be highest in the front-most dorsal nasal region, and decreased moving toward the posterior, where air flow rates and odor molecule levels decreased. According to the university, these findings reveal an intrinsically programmed configuration in the nose to ensure high sensitivity to odors.

Regarding potential applications, Ma explained to Perfumer & Flavorist, « Since primary cilia are present on almost every cell type in our body and brain, defects in cilia formation and function (ciliopathies) often cause complex symptoms involving brain malformation, obesity, cognitive deficits, cystic kidney disorder and blindness in addition to anosmia (unable to smell). In the long run, a better understanding of the factors mediating the olfactory cilia length and function may help to develop therapeutic means to treat cilia-related diseases. »

Publication

An Olfactory Cilia Pattern in the Mammalian Nose Ensures High Sensitivity to Odors

Rosemary C. Challis, Huikai Tian, Jue Wang, Jiwei He, Jianbo Jiang, Xuanmao Chen, Wenbin Yin, Timothy Connelly, Limei Ma, C. Ron Yu, Jennifer L. Pluznick, Daniel R. Storm, Liquan Huang, Kai Zhao, Minghong Ma

Current Biology, Volume 25, Issue 19, p2503–2512, 5 October 2015
DOI: http://dx.doi.org/10.1016/j.cub.2015.07.065

Highlights

  • Olfactory cilia length is correlated with odorant absorption in the nasal cavity
  • Cells with longer cilia have better access and higher sensitivity to odorants
  • The cilia length pattern is intrinsically programmed

Summary

« In many sensory organs, specialized receptors are strategically arranged to enhance detection sensitivity and acuity. It is unclear whether the olfactory system utilizes a similar organizational scheme to facilitate odor detection. Curiously, olfactory sensory neurons (OSNs) in the mouse nose are differentially stimulated depending on the cell location.

We therefore asked whether OSNs in different locations evolve unique structural and/or functional features to optimize odor detection and discrimination. Using immunohistochemistry, computational fluid dynamics modeling, and patch clamp recording, we discovered that OSNs situated in highly stimulated regions have much longer cilia and are more sensitive to odorants than those in weakly stimulated regions.

Surprisingly, reduction in neuronal excitability or ablation of the olfactory G protein in OSNs does not alter the cilia length pattern, indicating that neither spontaneous nor odor-evoked activity is required for its establishment. Furthermore, the pattern is evident at birth, maintained into adulthood, and restored following pharmacologically induced degeneration of the olfactory epithelium, suggesting that it is intrinsically programmed.

Intriguingly, type III adenylyl cyclase (ACIII), a key protein in olfactory signal transduction and ubiquitous marker for primary cilia, exhibits location-dependent gene expression levels, and genetic ablation of ACIII dramatically alters the cilia pattern. These findings reveal an intrinsically programmed configuration in the nose to ensure high sensitivity to odors. »


Sources:
http://www.perfumerflavorist.com/fragrance/research/Nasal-Cilia-The-Long-and-Short-of-Scent-sation-334826051.html?page=1
http://www.perfumerflavorist.com/fragrance/research/Nasal-Cilia-The-Long-and-Short-of-Scent-sation-334826051.html?page=2
http://www.cell.com/current-biology/abstract/S0960-9822%2815%2900933-1?_returnURL=http%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982215009331%3Fshowall%3Dtrue

Cet article n’engage que son auteur/ This article is the sole responsibility of the author

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